Coding

Part:BBa_K850003:Design

Designed by: Ellen Jorgensen   Group: iGEM12_NYU_Gallatin   (2012-10-02)


AGM1 and NAG1 genes from C.albicans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1442
    Illegal BglII site found at 1541
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cut the plasmid with Bba_K850000 with Spe1 and Pst and the plasmid with Bba_K850001 with Xba1 and Pst1, and gel-purified the insert which was cloned into the first plasmid.


Source

From combining Bba_K850000 and Bba_K850001

References